LC-MS calculators

Method scaling, mass accuracy and adducts, and dilutions. Results update as you type.

Scale a method to a new column

Keeps linear velocity and gradient column volumes the same. Same stationary phase assumed.

Current method

Column ID (mm) Length (mm) Particle size (µm) Flow (mL/min) Injection (µL) Gradient time (min)

New column

Column ID (mm) Length (mm) Particle size (µm)

Scaled method

Pressure and plate numbers are relative estimates at the same solvent, temperature, and packing quality. Check your system's pressure limit before running.

Mass error and adduct masses

Mass error (ppm)

Theoretical m/z Measured m/z XIC tolerance (ppm)

Adducts from neutral monoisotopic mass

Neutral mass M (Da)

Ion	m/z

Uses ion masses (electron mass accounted for). Verify against your own software before reporting values.

Dilution

Single dilution (C1 × V1 = C2 × V2)

Stock concentration Target concentration Final volume (µL)

Serial dilution

Top concentration Dilution factor (x-fold) Number of levels

Level	Concentration

Concentrations use whatever unit you enter. Keep stock and target in the same unit.

Estimates for planning. Confirm results on your instrument and follow your lab's SOPs.